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Muscle protein synthesis

Muscle protein synthesis (MPS) is the process by which muscle tissue is built from amino acids. Triggered most strongly by resistance training + a meal containing 25-40g of high-quality protein within 2 hours.

Updated May 2026 · 4 min read
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Definition

Muscle protein synthesis (MPS) is the process by which muscle tissue is built from amino acids. Triggered most strongly by resistance training + a meal containing 25-40g of high-quality protein within 2 hours.

What it means

MPS is regulated by mTOR signaling, which responds primarily to leucine concentration. The 'leucine threshold' for stimulating MPS is roughly 2.5-3g per meal — achieved by 25-40g of complete protein (whey, eggs, meat, fish, dairy, soy). Multiple meals/day beat one big protein bolus. The post-workout 'anabolic window' is real but wider than originally thought (2-4 hours).

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Why it matters

Spreading protein across 3-4 meals optimizes daily MPS more than concentrating it. People who eat all their protein at dinner don't get the same muscle-building stimulus as people who hit 25-40g at each meal. This is the actual reason for protein-distribution recommendations — not a vague 'metabolism' story.

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Frequently asked questions

How much protein per meal?

25-40g of complete protein for most adults; 40-50g for those over 60 (anabolic resistance increases with age).

Plant proteins enough?

Yes if you combine sources (rice + beans, soy + grains) to get a complete amino-acid profile, or use a single complete plant source like soy, quinoa, or buckwheat.

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